In the article beginning on page 1363 in the December 2004 issue, errors were identified in two figures.

In figure 2B, the panel showing Intralipid (40 µM) was incorrect due to image mismatching during panel preparation. The corrected panel B appears below.

In figure 4, in response to a question about the potential similarity of the bands in panel B, the authors noted that the bands are similar but not identical. All the immunoblots were performed in quadruplicate, and so the authors provided a new set of blots used for protein expression analysis from one of the quadruplicate experiments. This set of blots appears below (original figure appears on the left, with the corrected panel B on the right).

In panel C of figure 4, an incorrect actin blot was published. The corrected figure appears below (original figure appears on the left, with the corrected portion of panel C on the right).

The authors regret these errors in figure 2 and figure 4.

Fig. 2.

Comet assay of genomic DNA of rat astrocytes. Tail moments were quantified using Scion Image software. Astrocytes were treated for 24 h with propofol or a corresponding amount of Intralipid (40 µm, 80 µm, 160 µm, and 1 mm) (B). See original article for panels A, CG, and the full figure legend.

Fig. 2.

Comet assay of genomic DNA of rat astrocytes. Tail moments were quantified using Scion Image software. Astrocytes were treated for 24 h with propofol or a corresponding amount of Intralipid (40 µm, 80 µm, 160 µm, and 1 mm) (B). See original article for panels A, CG, and the full figure legend.

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Fig. 4.

Western blot analysis demonstrating caspase-3 activation in astroglial cells induced by SIN-1 (1, 2, and 3 mM) (A) and propofol (40 µm, 80 µm, 160 µm, and 1 mm) or Intralipid exposure (B). Astrocytes were exposed to SIN-1 (3 mm) for 18 h in the presence or absence of propofol (40 µm, 80 µm, 160 µm, and 1 mm) or Intralipid (C). The activation of caspase-3 was assessed by the presence of a 17-kd fragment derived from the cleavage of proenzyme caspase-3. Immunoblots are representative of four independent experiments. SnMP = tin mesoporphyrin. The original figure appears on the left, with the corrected panels on the right.

Fig. 4.

Western blot analysis demonstrating caspase-3 activation in astroglial cells induced by SIN-1 (1, 2, and 3 mM) (A) and propofol (40 µm, 80 µm, 160 µm, and 1 mm) or Intralipid exposure (B). Astrocytes were exposed to SIN-1 (3 mm) for 18 h in the presence or absence of propofol (40 µm, 80 µm, 160 µm, and 1 mm) or Intralipid (C). The activation of caspase-3 was assessed by the presence of a 17-kd fragment derived from the cleavage of proenzyme caspase-3. Immunoblots are representative of four independent experiments. SnMP = tin mesoporphyrin. The original figure appears on the left, with the corrected panels on the right.

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1.
Acquaviva
R
,
Campisi
A
,
Murabito
P
et al
:
Propofol attenuates peroxynitrite-mediated DNA damage and apoptosis in cultured astrocytes: An alternative protective mechanism.
Anesthesiology
2004
;
101
:
1363
71