Fig. 3. Dependence of human liver microsomal malondialdehyde (MDA) production on halothane concentration. Reactions were performed as described in Materials and Methods, with varying halothane concentration (0–2 μl, producing a concentration of 0–3.5% (v/v). Data points represent observed values. Lines represent values predicted from parameters determined by nonlinear regression analysis using a two-enzyme model. CTE = chlorotrifluoroethane; CDE = chlorodifluoroethene.