Fig. 3. AnnexinV/propidium iodide staining was performed to assess phosphatidylserine translocation from the inner to the outer cell surface. Myocytes grown on coverslips were exposed to norepiniphrine (NE; 10 μm) alone or in presence of diltiazem (DIL; 1 μm), propranolol (PRO; 2 μm), isoflurane (ISO; 1.6 MAC), and halothane (HAL; 1.2 MAC) for 12 h and subjected to annexinV/propidium iodide staining as described in Methods. (A ) Mean percentages of adherent cells with the early apoptotic pattern of labeling (AN+/PI−) as well as the late apoptotic pattern of labeling (An+/PI+). Data are mean ± SD. *P < 0.0001 versus control (CTL), †P < 0.0001 versus NE, not significant versus CTL. (B ) Early apoptotic pattern of annexinV/propidium iodide staining: An+/PI−. (C ) Late apoptotic pattern of annexinV/propidium iodide staining: An+/PI+ (bar = 25 μm).