Fig. 1. Hemodynamic performance of isolated rat hearts during 1 h of perfusion: Control group perfused with Krebs-Henseleit solution (KH), KH > protamine > KH group treated with protamine from 20 to 40 min, KH + anti-TNF > protamine + anti-TNF > KH + anti-TNF group treated with anti-TNF antibodies at all perfusion time, KH + heparin > protamine + heparin > KH + heparin group treated with heparin at all perfusion time, KH > protamine > KH + heparin group treated with heparin at time of washout (last 20 min). Results are presented as the percentage of baseline measurements for each heart. No significant differences found between control group and groups treated with anti-TNF antibodies or heparin throughout perfusion. *P < 0.05 versus  control group. **P < 0.05 for measures on 60 versus  40 min for KH > protamine > KH + heparin group.