Fig. 4. Representative tracing showing results from the protocol used to determine effect of hexanol on the relation between force and intracellular [Ca2+]iin the presence of muscarinic stimulation provided by acetylcholine. The upper panel shows changes in F340:F380(an index of intracellular [Ca2+]i), and the lower panel shows isometric force. After depletion of internal calcium stores and determination of the response to incremental increases in CaCl2, the effect of hexanol is determined. The dashed lines show that the response to 1 mm CaCl2after hexanol washout was similar to that measured before hexanol exposure.