Fig. 3. Dose–response curve of excitatory amino acid transporter type 3 in the presence or absence of isoflurane or phorbol 12-myrisate 13-acetate (PMA). Oocytes were exposed to isoflurane (0.70 mm for 3 min) or PMA (100 nm for 10 min). Inset graph is a Lineweaver-Burk plot using the mean value of the respective groups with abscissa for reciprocal of l-glutamate concentration (micromolars) and ordinate for reciprocal of response (microcoulombs). r2= 0.99 for the control, isoflurane, or PMA group. *P < 0.05 compared with control.