Fig. 6. Cell survival (A ) and lactate dehydrogenase (LDH) release (B ) in rat vascular smooth muscle cells after immediate or delayed (12 h or 24 h) isoflurane pretreatment in absence or presence of KATPinhibitors (glybenclamide, 5-hydroxydecanoate [5-HD], or 1-[5-[2-(5-chloro-o-anisamido)ethyl]-2-methoxyphenyl]sulfonyl-3-methylthiourea [HMR-1098]). Asterisk denotes that KATPinhibitors abolished the protective effects of isoflurane. Number sign denotes that KATPinhibitors significantly attenuated the protective effects of isoflurane pretreatment. Data are mean ± SD (n = 6). Significance was considered as P < 0.05.