![Fig. 4. (A–E ) Representative traces of [CA2+]cyt-changes with sequential stimulation using 100 mm KCl followed by 1 mm carbachol (CAB). (A ) Control. (B–F ) Cells were pretreated for 5 min with 10 mm caffeine (CAF). (B ), 1 μm thapsigargin (TG) (C ), 10 mm CAF and 1 μm TG (D ), 10 mm CAF and 1 μm CAB (E ), or with 1 μm TG and 1 μm CAB (F ). (G, H ) Statistical summary. Values presented are mean ± SEM (n = 2–19). The significance of the differences among different groups was tested using the Student-Neumann-Keuls test and labeled as follows:*P < 0.05 to the control group; $P < 0.05 to the TG group.](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/100/5/10.1097_00000542-200405000-00062/2/62ff1.png?Expires=1717073636&Signature=j0KFRNW326Ed9rcRfMNFjoSePsu6fqedl1btq41Z7e13ooGE48SV9VfIhkBSJO3xd-ACqPLmGDX0rDf5UvOt1~1VbRGjubSDmNMUi95yL9aBQ9AkU5rmZRfcK2~9weiwOpH5t3YGtJlXd-nhCFIic0o1N0UKfATSF6~AvfTu7dbXrmE06V8vhDSvn5Yx~mYqxS0xEebYnOr39BTh7uu-ZlOfLLZ1mEYUWVBa52dNahp88JxvDGwPEc0d5zNGb-aJ8HcmvGyg3o5x3G96YrIKVmlVzAkF4dPoFwW-i6qVDi6jf7WaUALp6RbowAnwqr6oLJSRF5ae68LglJk0bME82A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fig. 4. (A–E ) Representative traces of [CA2+]cyt-changes with sequential stimulation using 100 mm KCl followed by 1 mm carbachol (CAB). (A ) Control. (B–F ) Cells were pretreated for 5 min with 10 mm caffeine (CAF). (B ), 1 μm thapsigargin (TG) (C ), 10 mm CAF and 1 μm TG (D ), 10 mm CAF and 1 μm CAB (E ), or with 1 μm TG and 1 μm CAB (F ). (G, H ) Statistical summary. Values presented are mean ± SEM (n = 2–19). The significance of the differences among different groups was tested using the Student-Neumann-Keuls test and labeled as follows:*P < 0.05 to the control group; $P < 0.05 to the TG group.
