Fig. 3. Change in flavoprotein oxidation during treatment with and after washout of diazoxide and isoflurane. (A ) Representative trace of the change in flavoprotein fluorescence by treatment with 50 μm diazoxide [Dz (+)] followed by removal of diazoxide [Dz (−)]. Dinitrophenol (DNP; 1 mm) was added to maximally activate flavoprotein oxidation. The data were expressed as a percentage of 1% dinitrophenol–induced flavoprotein oxidation. (B ) Representative trace of the change in flavoprotein fluorescence by treatment with 2% isoflurane [Iso (+)] followed by removal of isoflurane [Iso (−)]. (C ) Quantitative analysis of flavoprotein oxidation during and after treatment with diazoxide and isoflurane. Each bar  represents the mean ± SEM of seven experiments.