Fig. 5. Alveolar macrophage distribution during mechanical ventilation. Alveolar macrophages were stained with mouse anti-rat macrophage ED-1 antibody. Markedly fewer macrophages were seen in alveolar space after ventilation with tidal volume of 40 ml/kg and zero positive end-expiratory pressure (PEEP) compared with those ventilated with tidal volume of 7 ml/kg and 3 cm H2O PEEP at 60 and 120 min. * P < 0.05 versus no ventilation at the identical compartment, respectively.
