Fig. 2. Typical example of a Western blot showing the effects of anesthetics on total tyrosine phosphorylation ( upper panel ; left row = control [100%]) and specific focal adhesion kinase (pp125FAK) phosphorylation ( lower panel ) in rat hippocampal slices. The concentrations of anesthetics used in this experiment (corresponding fractional increases from basal phosphorylation, respectively) were 0.3 mm (270%) for desflurane, isoflurane (220%), and sevoflurane (295%), 5 μm (270%) for thiopental, for etomidate (235%) and for propofol (208%). Ketamine concentration was 10−4m.