Fig. 1. The effects of pancuronium on glucocorticoid-responsive element (GRE)–mediated transcription. C2C12 myoblasts were transfected with glucocorticoid receptor expression vector, GRE-linked firefly luciferase, and control sea pansy luciferase. GRE-mediated transcription was evaluated by GRE-driven firefly lucif-erase activity that was normalized to sea pansy luciferase activity and was expressed as percent control (Cont). The figure shows the representative results of three independent experiments, each of which were performed in triplicate. *P < 0.005 versus  control; **P < 0.002 versus  control. Dexamethasone (Dex) induced GRE-mediated transcription, whereas pancuronium did not alter GRE-mediated transcription. Dantrolene (D) and d-tubocurarine (T) showed no effects on GRE-mediated transcription, either.