Fig. 2. Semiquantitative reverse transcriptase polymerase chain reaction of proinflammatory markers keratinocyte derived chemokine (KC) ( A ), macrophage inflammatory protein 2 (MIP-2) ( B ), intracellular adhesion molecule-1 (ICAM-1) ( C ), and monocyte chemoattractant protein 1 (MCP-1) ( D ) from renal cortices of sham operated C57 control mice, C57 mice subjected to cecal ligation and double puncture (CLP) with saline infusion, C57 mice subjected to CLP while receiving infusion of 10% lidocaine, and C57 mice treated with infusion of 1% bupivacaine. Bar graphs represent densitometric quantifications of relative band intensities from reverse transcriptase polymerase chain reactions for each indicated mRNA, and are compilations of at least three separate experiments. A representative image of the semiquantitative reverse transcriptase polymerase chain reaction gels for each gene with sham, saline CLP, and 10% lidocaine with CLP is also shown. bp = basepair; M = 100 basepair DNA marker; Bupiv = bupivacaine; Lido = lidocaine. # P < 0.05 versus sham; *P < 0.05 versus saline vehicle .