Fig. 6. Protein oxidation in N2 and gas-1 . Western blot of mitochondrial proteins probed with an antibody to derivatized carbonyl groups ( A ) or with an antibody specific for 4-hydroxy-2-nonenal Michael adducts ( B ). A similar band of approximately 26 kd is identified by both techniques ( arrow ). Each lane of gas-1 represents a separate isolation of mitochondria, indicating that the increased labeling of the 26-kd protein is consistent. Similar results were obtained in three different experiments.