Fig. 2. Effects of pretreatment with the protein kinase C-ε antagonist PP93 and scrambled protein kinase C-ε peptide PP105 on the activation of sarcolemmal adenosine triphosphate–sensitive potassium channel by isoflurane (Iso). The voltage protocol was similar to the one described in figure 1 . ( A ) Effect of the antagonist peptide. PP93 (200 nm) was added to the external solution 5 min before adding the protein kinase C-ε agonist PP106 (200 nm). ( B ) Effect of the scrambled protein kinase C-ε peptide. PP105 (200 nm) was added to the external solution before adding isoflurane. Isoflurane (0.88 mm) did not elicit the sarcolemmal adenosine triphosphate–sensitive potassium current (IKATP) after pretreatment with either the antagonist or the scrambled peptide. Similar results were observed in n = 7/group. Pinacidil (Pin, 20 μm) was used to confirm the availability of functional sarcolemmal adenosine triphosphate–sensitive potassium channels.