Fig. 1. ( A ) Protocols for physiologic, histologic and biochemical analyses. Sampling for WB = sampling whole hearts for Western blotting; Sampling for ISH = sampling whole hearts for in situ hybridization; PKC inhibitor = protein kinase C inhibitor, bisindolylmaleimide. Groups: ( I ) = Time-control; ( II ) = 30-min propofol infusion; ( III ) = 3-min regional ischemia and 90-min reperfusion; ( IV ) = 3-min regional ischemia and 90-min reperfusion including 30-min propofol infusion; ( V ) = a PKC inhibitor and 3-min regional ischemia and 90-min reperfusion including 30-min propofol infusion. ( B ) Representative left ventricular (LV) pressure (P)-volume (V) loops in an ejecting in situ rat heart during an aortic occlusion. Solid circles = end-systolic P-Vdata on the enveloping curve; ESPVR = end-systolic pressure-volume relation; SV = stroke volume (EDV − ESV); Ea = effective arterial elastance; ESP/SV. ( C ) a representative ESPVR. mLVV = midrange LVV; ESPmLVV = ESP at mLVV; PVAmLVV = systolic pressure-volume area at mLVV. Volume axes indicate normalized absolute LV volume for 1 g LV mass in B and C .