Fig. 7. Representative immunoblot of intercellular adhesion molecule (ICAM)-1 in renal cortex of sham, pentobarbital-treated, and sevoflurane-treated (as a representative anesthetic) rats. A single protein band of an apparent 60-kd molecular mass was detected using monoclonal ICAM-1 antibody. Sevoflurane anesthesia (Sevo) during ischemia and reperfusion (IR) reduced ICAM-1 protein expression compared with pentobarbital (PB)–anesthetized rats subjected to IR.  Sham represents kidney from sham-operated rats, and  contralat represents kidneys contralateral to the ones subjected to IR. Representative of five independent experiments.