Fig. 5. One representative Western blot experiment of cytosolic fraction showing time-course of ERK1 phosphorylation during desflurane-induced preconditioning. ( Top ) phosphorylated form of ERK1; ( bottom ) total ERK1. The histogram presents densitometric quantification of four experiments as x-fold average light intensity. Data are presented as mean ± SD; ** P < 0.01 versus vehicle baseline; * P < 0.05 versus vehicle baseline; † P < 0.05 versus Calph C baseline.