Fig. 6. One representative Western blot experiment of cytosolic fraction showing time-course of ERK2 phosphorylation during desflurane-induced preconditioning. (  Top ) phosphorylated form of ERK2; (  bottom ) total ERK2. The histogram presents densitometric quantification of four experiments as x-fold average light intensity (AVI). Data are presented as mean ± SD; **  P < 0.01  versus vehicle baseline; *  P < 0.05  versus vehicle baseline; †  P < 0.05  versus Calph C baseline.