Fig. 3. Western blot analysis of phosphorylation of Akt at Ser 473 (  top lanes ) and total Akt (  bottom lanes ) in rabbits randomly assigned to receive 0.9% saline (CON), dimethyl sulfoxide (DMSO), wortmannin (0.6 mg/kg, intravenously), 1.0 minimum alveolar concentration isoflurane administered for 3 min before and 2 min after reperfusion in the presence and absence of wortmannin pretreatment, or the combination of postconditioning ischemia (10 s) and 0.5 minimum alveolar concentration isoflurane in the presence and absence of wortmannin pretreatment. Histograms depicting the relative density of phospho-Akt in each experimental group are shown in the  lower panel . *Significantly (  P < 0.05) different from CON.