Fig. 3. Caspase-3 processing, poly-ADP-ribose-polymerase (PARP) cleavage, and caspase-3–like activity in response to various concentrations of desflurane, isoflurane, and sevoflurane. ( A – C ) Anti–caspase 3 ( upper panel ) and anti-PARP ( lower panel ) Western blot analysis of the cytosols of Jurkat T lymphocytes exposed to the indicated doses of anesthetics. p32: inactive caspase-3 proform; p19/p17: processed, active caspase-3 fragments. The data shown are representative of six independent experiments. ( D ) Fluorogenic caspase-3 assay (DEVDase assay) of the above cytosols. The results are given in arbitrary fluorescent units (RFUs). Data are presented as the median and 25–75% and 95% confidence intervals of 10 independent experiments. * P < 0.05 versus untreated cells.