Fig. 4. Effect of the general caspase inhibitor Z-VAD.fmk on sevoflurane-induced apoptosis in Jurkat T lymphocytes. ( A – C ) Flow cytometric analysis of green fluorescent protein (GFP)-annexin V and propidium iodide staining of Jurkat T lymphocytes after sevoflurane exposure (8 vol% for 24 h) in the absence or presence of Z-VAD.fmk (100 μm). ( Insets ) Percentage of cells for each quadrant. ( D ) Anti–caspase-3 ( upper panel ) and anti–poly-ADP-ribose-polymerase (PARP; lower panel ) Western blot of MG132 (1 μm)– and sevoflurane-treated Jurkat T cells in the absence or presence of Z-VAD.fmk. The data shown are representative of six independent experiments.