Fig. 4. Example of light micrographs of  ex vivo rat liver slides (ischemia-reperfusion) stained with PAS at ×100 magnification. Top panels (  A and  B ) refer to fed rats;  A at 0 min and  B at the end of the experiment,  i.e., 135 min. Bottom panels (  C and  D ) refer to fasting animals,  C at 0 min and  D at 135 min. Box panels display the glycogen content analyzed using the NIH-Image software (National Institutes of Health, Bethesda, MD, USA) at the same time-points.