Fig. 6. Representative immunohistochemistry photomicrographs, corresponding histograms, and representative Western blots depicting phospho-Akt ( A ) and Bcl-2 ( B ) expression in neonatal rat ventricular myocytes under control (CON) conditions, after exposure to hydrogen peroxide (H2O2), and after pretreatment with isoflurane in the presence (ISO + H2O2) or absence (ISO) of hydrogen peroxide. * Significantly ( P < 0.05) different from CON; † significantly ( P < 0.05) different from H2O2, n = 4. Western blotting was repeated at least twice, with similar results as in the blots shown in the figures.