Fig. 3. Representative photomicrographs of immunofluorescent staining for endothelial nitric oxide synthase (eNOS;  top panel ) and histograms illustrating eNOS expression (  bottom panel ) in left ventricular tissue samples obtained from rabbits before (control), immediately after (trigger), or 24 h after (mediator) prolonged (2 h) administration of 1.0 minimum alveolar concentration isoflurane in the absence (−) and presence (+) of  N -nitro-l-arginine methyl ester (l-NAME). * Significantly (  P < 0.05) different from control. † Significantly (  P < 0.05) different from (−) l-NAME. 

Fig. 3. Representative photomicrographs of immunofluorescent staining for endothelial nitric oxide synthase (eNOS;  top panel ) and histograms illustrating eNOS expression (  bottom panel ) in left ventricular tissue samples obtained from rabbits before (control), immediately after (trigger), or 24 h after (mediator) prolonged (2 h) administration of 1.0 minimum alveolar concentration isoflurane in the absence (−) and presence (+) of  N -nitro-l-arginine methyl ester (l-NAME). * Significantly (  P < 0.05) different from control. † Significantly (  P < 0.05) different from (−) l-NAME. 

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