Fig. 3. Infarct size was determined using 1% triphenyltetrazolium chloride staining, as described in the Materials and Methods section. In each group, five hearts were used. Data are given as mean ± SD. ATR = atractyloside (10 μm in < 0.1% DMSO); CTRL = time-matched perfusion; ISCH = unprotected hearts exposed to ischemia-reperfusion; LY = LY294002 (15 μm in < 0.1% DMSO); PostC = anesthetic postconditioning. * P < 0.05 versus ISCH.