Fig. 5. (  A ) Representative trace demonstrating the effect of bisindolylmaleimide I (Bis; 10 μm) and Bis plus propofol (30 μm) on myocyte shortening and intracellular free Ca²⁺concentration in a single ventricular myocyte during α^1a-adrenoreceptor activation with phenylephrine (PE; 10 μm). (  B ) Summarized data for the concentration-dependent effects of propofol during α^1a-adrenoreceptor activation on myocyte shortening and intracellular free Ca²⁺concentration in the presence of protein kinase C inhibition with Bis. Results are expressed as percent of baseline value. Control (Ctrl) represents the steady state baseline value for twitch amplitude or the change in intracellular free Ca²⁺concentration, set to 100%. *  P < 0.05 compared with control; #  P < 0.05 compared with phenylephrine. n = 18 cells from 7 hearts.