Fig. 3. Immunohistochemical staining of vascular endothelial growth factor in lungs. ( A ) Stained with unimmunized rabbit serum as the primary antibody. ( B ) Lungs of a rat anesthetized with 2.5% sevoflurane for 4 h. ( C – F ) Lungs of rats anesthetized with 1.5% isoflurane for 4 h. Horizontal bars below the figures indicate 100 μm.