Fig. 4. Method to analyze the effect of sevoflurane on the postsynaptic γ-aminobutyric acid type A receptor response. The graph shows the response of the neuron in  figure 3 to picoejection of the γ-aminobutyric acid type A agonist muscimol at increasing dose rates. Linear regression analysis was performed with the y-intercept constrained to pass through the neuronal control frequency at the zero dose rate. The anesthetic effect in terms of neuronal frequency is illustrated by interpolation of the net decrease at an identical dose rate,  e.g. , at 0.05 pmol/min muscimol, from the dose–response curves at 0 minimum alveolar concentration (MAC) (  solid line ,  filled circles ) and 1 MAC (  dashed line ,  open circles ). Sevoflurane caused an enhancement of the muscimol-induced net decrease from 40.9 to 59.4 Hz.