Fig. 4. The effect of pretreatment with  S (+)- or  R (−)-ibuprofen (1.0 μm; IBU) on prostaglandin E²(PGE²)–evoked glutamate release. Spinal (lumbar) slices were harvested 3 days after spinal nerve ligation or sham surgery. Each  point represents the mean ± SEM of at least 6 animals (4–10 slices/dose). DMSO = dimethyl sulfoxide.