Fig. 6. Western blots analysis. Phosphorylation status of protein kinase B/Akt (PKB, 60 kd, phosphorylation at Ser-473) ( A ), glycogen synthase kinase 3β (GSK3β, 46 kd, phosphorylation at Ser-9) ( B ), endothelial nitric oxide (eNOS, 140 kd, phosphorylation at Ser-1177) ( C ), ribosomal S6 kinase (p70S6K, 70/85 kd, phosphorylation at Thr-389) ( D ), extracellular signal–regulated kinase 1/2 (ERK1/2, 44 and 42 kd, phosphorylation at Thr-202/Tyr-204), p38 mitogen-activated protein kinase (p38 MAPK, 43 kd, phosphorylation at Thr-180/Tyr-182), and protein kinase C ϵ (PKC-ϵ, 95 kd, phosphorylation at Ser-729) ( E ) were analyzed with specific phospho-antibodies. CTRL = time-matched perfusion; ISCH = unprotected remodeled hearts exposed to ischemia/reperfusion alone; LY = LY 294002 (15 μm); PostC = anesthetic postconditioning. Data are given as mean ± SD (n = 5 in each group). * P < 0.05 PostC versus ISCH.