Fig. 5. Examples of representative propidium iodide (PI) fluorescence images of hippocampal slices from 5-day-old and 23-month-old rats in oxygenated media (oxygen/control), after exposure to isoflurane for 20 min, after 20 min of oxygen and glucose deprivation (OGD), and after a combination of isoflurane and OGD for 20 min.