Fig. 2. Neuroprotection afforded by sevoflurane was assessed by the reduction of the increase in lactate dehydrogenase (LDH) release and the decrease in the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction, both induced by oxygen–glucose deprivation (OGD). (  A ) Neuron death after 90 min of OGD was assessed by LDH release to the bathing medium. (  B ) Neuronal viability was assessed 24 h after 90 min of OGD by the densitometric analysis of MTT reduction test. Results were expressed as mean percentage of the optical density measured in sham wash cells. n = 24; *  P < 0.05  versus OGD cells.