Fig. 1. Effect of 10 μm nondepolarizing neuromuscular blocking agents (NMBAs) on acetylcholine (ACh)–mediated responses in voltage clamped (−60 mV) Xenopus oocytes expressing human neuronal nAChRs. ( A ) Representative traces showing the inhibitory effect of 10 μm rocuronium (Roc) on the α3β2, α3β4, α4β2, and α7receptor subtypes (displayed in the same order). ( B ) Responses in each oocyte were normalized to the maximal acetylcholine peak current and maximal net charge (α7) in each oocyte, giving the concentration–response curves. Each symbol represents mean ± SEM of 3–14 oocytes. When no error bars are seen, they are smaller than the symbols.