Fig. 3. Neutrophil apoptosis in bronchoalveolar lavage. (  A ) Gating of neutrophils with Ly6. (  B ) Apoptotic neutrophils stained with the terminal deoxynucleotidyl transferase-mediated nick end-labeling procedure. (  C ) Apoptotic neutrophils on days 7 and 14 in control (C) and overventilated (OV) lungs after 120 and 240 min of perfusion and ventilation. Data are mean ± SEM from 3–7 animals/group.