Fig. 4. Effect of depletion of natural killer cells or depletion of natural killer cells and natural killer T cells on the number of liver metastases 12 days after EL4 cell injection. Values are expressed as percentage of the control value (± SD). Both anti-AGM1 and anti-NK1.1 treatment significantly increased the number of metastases compared with the control, sevoflurane + laparotomy, and sevoflurane + spinal + laparotomy groups (**). Between the two drug treatment groups, the difference was statistically significant (**).  Bars denote the mean value of four animals in the drug treatment groups and eight animals in the other groups;  error bars = SD. **  P < 0.01.