Fig. 3. Effects of hemin on type 2 cationic amino acid transporter (CAT-2) expression and CAT activity in lipopolysaccharide (LPS)–stimulated murine macrophages. Representative gel photography illustrates the products of immunoblotting assay. The CAT-2 protein concentrations were normalized by β-actin. The CAT activity was determined by measuring the l-arginine transport using l-[3H]arginine uptake assay. Data are expressed as mean ± SD. Hemin (5) = 5 μm hemin; Hemin (50) = 50 μm hemin; Hemin (500) = 500 μm hemin; PBS = phosphate-buffered saline. * P < 0.05 compared with the negative control group. # P < 0.05 compared with the positive control group. † P < 0.05, LPS + Hemin (500) or LPS + Hemin (50) group versus LPS + Hemin (5) group. ‡ P < 0.05, LPS + Hemin (500) group versus LPS + Hemin (50) group.