Fig. 6. Immunolocalization of protein kinase C (PKC)-ι. In unstimulated cells, PKC-ι exhibited a cytoplasmic fluorescent pattern, which was especially abundant in the perinuclear zones (  A ). No redistribution of PKC-ι was observed in cells exposed to phorbol 12-myristate 13-acetate (  B ). In acetylcholine-treated cells, the cytoplasmic staining pattern was similar to that of unstimulated cells (  C ).