Fig. 6. (  A –D ) Currents through human  ether-a-go-go –related gene (HERG) wild-type (wt) and mutant channels under control conditions and after application of 300 μm bupivacaine. The first 250 ms of the deactivating tail currents are shown as  blow-up . The respective pulse protocols are shown as  insets . (  E and  F ) Comparison of the residual current I^bupivacaine/I^controlafter application of 300 μm bupivacaine for HERG wild-type and the different mutant channels. Inhibition by 300 μm bupivacaine was reduced by all mutations (  P < 0.001). n.d. = no data.