Fig. 6. ( A ) Representative Western blot for nitric oxide synthase (NOS) 1 in isolated cardiomyocytes from healthy and diabetic rats. ( B ) Coimmunoprecipitation NOS1/Cav-3 from cardiomyocyte extracts of healthy and diabetic rats. IP = immunoprecipitate; S. = supernatant fraction. ( C ) Bar graphs displaying total activity using N G-nitro-l-arginine methylester (l-NAME; nonselective NOS inhibitor) and percentage NOS activity inhibited by vinyl-l- N -5-(1-imino-3-butenyl)-l-ornithine (l-VNIO; NOS1 inhibitor) in isolated cardiomyocytes from healthy and diabetic rats. Results are expressed as mean ± SD (n = 5 in each group). * P < 0.05 versus healthy group.