Fig. 6.
Confocal image of a nerve bundle for nerve growth factor (NGF) and S100 fluorescent double-labeling immunohistochemistry. (A) NGF fluorescent immunohistochemistry in unincised skin. (B) S100 fluorescent immunohistochemistry for Schwann cells (green) in unincised skin. (C) Merged image shows only S100-positive staining in nerve bundle in unincised skin. (D) NGF fluorescent immunohistochemistry (red) from nerve bundle in incised skin. (E) S100 immunohistochemistry for Schwann cells (green) in incised skin. (F) Merged image of NGF fluorescent (red) and S100 fluorescent (green) immunohistochemistry. Colocalized cells (yellow) are indicated byarrows. Some NGF-positive, S100-negative structures are present (arrowheads).Scale bars: 50 μm forA–C; 10 μm forD–F.