Fig. 7. Nitrotyrosine containing protein levels were measured by Western blot in 50 μg of kidney homogenates. Densitometric values were normalized to actin and are expressed as a ratio of sham ± SD, with n = 4 each in the sham and saline control groups and n = 6 each in the PI dextrose and PR dextrose groups. Nitrotyrosine containing protein levels did not change in the saline group after ischemia-reperfusion compared with sham but were significantly increased in the PI dextrose group. *  P < 0.05  versus sham. #  P < 0.05  versus saline. $  P < 0.05  versus PR dextrose. Nitrotyrosine containing protein levels in the PR dextrose group were similar to those in saline controls. PI dextrose = hyperglycemia lasts through the duration of ischemia; PR dextrose = hyperglycemia begins 2 h after initiation of reperfusion.