Fig. 2. Cannabinoid receptor type 2 (CBR2) staining and localization. Representative confocal images of CBR2 ( red ), ED2/CD163 (ED2; green ) and 4′,6-diamidino-2-phenylindole dihydrochloride hydrate (DAPI; blue in A ) or ionized calcium binding adaptor molecule 1 (Iba1; green in B ) staining of superficial laminae of dorsal horn ipsilateral to L5 nerve transection surgery. The first three columns show individual staining of these markers, and the fourth column depicts the merge of all the markers showing coregionalization of CBR2 with perivascular (ED2) and microglial (Iba1) cells.