Fig. 1. Western blotting and immunofluorescence analysis of NEP1-40 transduction in the brains of TAT-β-Gal–, No-TAT-NEP1-40–, and TAT-NEP1-40–treated animals (n = 3). (A ) Western blotting shows the high levels of β-Gal protein at approximately 120-kd position in the TAT-β-Gal–treated animals (lane 1 ) and NEP1-40 protein at 14.4-kd position in the TAT-NEP1-40–treated animals (lane 3 ). Line 2 is the blotting result of No-TAT-NEP1-40–treated animals. (B ) Representative microphotographs showing the immunofluorescence staining in the brains of TAT-β-Gal (a and d )–, TAT-NEP1-40 (c and f )–, and No-TAT-NEP1-40 (b and e )–treated animals. Scale bar = 100 μm; same magnification is used in all parts of B . a , b , and c = the ipsilateral hemisphere to ischemia; d , e , and f = the contralateral hemisphere to ischemia. β-Gal =β-galactosidase; NEP1-40 = Nogo extracellular peptide 1-40; TAT = trans-activator of transcription.