Fig. 4. Western blot analysis of myocardial Ca2+/calmodulin-dependent protein kinase (CaMK) IIΔcontent at early reperfusion after blockade of β-adrenergic receptors ( A ) and downstream kinases protein kinase A (PKA) and CaMK IIΔ( B ) and at late reperfusion after continuous blockade of β-adrenergic receptors ( C ). Rabbits received vehicle (CON), desflurane (DES), esmolol for 30 min (ESMO 30) or 180 min of reperfusion (ESMO 180), and ICI 118,551 (ICI), H-89, or KN-93 alone or in combination with desflurane. Results are presented as representative original immunoblottings and average densitometric results of immunoblotting as percentage of control (n = 5 per group). Note that data from Sham, CON, and DES in B are the same as in A . Values are mean ± SEM.