Fig. 7. Metallothioneins-I/II (MT-I/II) localize to neuronal cell bodies in cortical neuronal cultures. MT-I/II localization was visualized by epifluoresence microscopy. Cultures 10 days  in vitro were labeled using a monoclonal antibody against MT-I/II and a polyclonal antibody against microtube-associated protein-2 (MAP2). Secondary labeling was accomplished with Alexafluor 488 or Alexafluor 594 fluorescent conjugates. (  A ) WT 129 cells, nuclei were stained with Hoechst. (  B ) MT-I/II knockout cells. (  C ) WT 129 cultures, antibody preincubated with 10 μm horse MT-I/II protein to show MT antibody specificity.