Fig. 4.
Responses of Ca2+ transient measures to different Ca2+ channel subtype blockers in small (A) and large (B) control sensory neurons stimulated by high K+. Mibefradil (Mib, 200 nm), nicardipine (Nic, 10 μm), SNX-111 (“111,” 200 nm), ω-agatoxin-IVA (Aga, 200 nm), and SNX-482 (“482,” 100 nm) were applied. Each neuron was exposed to only a single agent. Anasteriskwithin thebarindicates a significant effect on the transient after application compared with baseline. There was a significant main effect between agents for each measure in each cell size category.Bracketsindicate significant differences bypost hocpaired comparisons (Bonferroni). Area = transient area; R = ratio of fluorescence excited by 340 nm divided by fluorescence excited by 380 nm; RBLΔ = offset of the posttransient baseline above the original baseline; RΔmax = transient amplitude. Numbers in parentheses (n) apply to each parameter for that neuronal size. Note that the scales are different in the positive and negative direction for Area in large neurons.