Intrathecal pretreatment of rats with small interfering RNA (siRNA)311 inhibits the enhanced surface delivery of GluR1 after plantar incision. (A) Representative Western blots and quantitative analysis of band density show GluR1 and GluR2 protein levels in the plasma membrane fraction of ipsilateral dorsal horn tissue from different groups. β-tubulin was used as an internal reference. Pretreatment of rats with intrathecal siRNA311, but not scrambled (Scr) siRNA or vehicle, prevented the increase in GluR1 expression in the plasma membrane fraction of ipsilateral dorsal horn at 3 h after plantar incision. (B) Representative Western blots and quantitative analysis of band density show GluR1 and GluR2 protein levels in the cytosolic fraction in different groups. *P < 0.05 compared with the control group. n = 6 rats per group. Ctrl = control (naive) group.
