Fig. 8.
Effect of propofol on hERG channels. (A) Time course of hERG tail current recorded in a typical experiment in the absence and the presence of 30 and 100 μM propofol with the original hERG traces at corresponding time points shown. (B) Voltage-dependent hERG current was recorded in a representative cell with the voltage protocol shown in the inset and was reversibly inhibited by 30 and 100 μM propofol. (C) Current–voltage relation of hERG tail current in the absence and presence of 30, 100, and 300 μM propofol (n = 10 for 30 and 100 μM, and n = 7 for 300 μM, P < 0.05, 30 μM vs. control at +20 to +60 mV; *P < 0.05 or **P < 0.01, 100 or 300 μM vs. control at 0 to +60 mV, ANOVA). (D) Current–voltage relation of hERG step current in the absence and presence of 30, 100, and 300 μM propofol (n = 10 for 30 and 100 μM, and n = 7 for 300 μM, P < 0.05, 30 μM vs. control at +20 to +60 mV; *P < 0.05 or **P < 0.01, 100 or 300 μM vs. control at 0 to +60 mV, ANOVA). (E) Concentration–response relation for inhibiting hERG current (at +40 mV) by propofol fitted with a Hill equation (n = 7). (F) Mean values of g/gmax were calculated with hERG tail current and fitted with a Boltzmann function in the absence and the presence of 30 and 100 μM propofol.