Fig. 7.
Effects of allopregnanolone sulfate (APAS) on inactivation curves in oocytes expressing Nav1.2 (A) (n = 6), Nav1.6 (B) (n = 7), Nav1.7 (C) (n = 5), or Nav1.8 (D) (n = 6) α subunits with β1 subunits. Currents were elicited by a 50-ms test pulse to −20 mV for Nav1.2 and Nav1.6, −10 mV for Nav1.7, and +10 mV for Nav1.8 after 200 ms (500 ms for only Nav1.8) prepulses ranging from −140 mV to 0 mV in 10-mV increments from a Vmax holding potential. Representative INa traces in both the absence and presence of APAS are shown in A-1, B-1, C-1, and D-1. Effects of APAS on inactivation curves (closed circles, control; open circles, neurosteroids; cross, washout) are shown in A-2, B-2, C-2, and D-2. Steady-state inactivation curves were fitted to the Boltzmann equation, and the V1/2 values are shown in table 2. Data are expressed as means ± SEM. Nav = voltage-gated sodium channel; Wash = washout.

Effects of allopregnanolone sulfate (APAS) on inactivation curves in oocytes expressing Nav1.2 (A) (n = 6), Nav1.6 (B) (n = 7), Nav1.7 (C) (n = 5), or Nav1.8 (D) (n = 6) α subunits with β1 subunits. Currents were elicited by a 50-ms test pulse to −20 mV for Nav1.2 and Nav1.6, −10 mV for Nav1.7, and +10 mV for Nav1.8 after 200 ms (500 ms for only Nav1.8) prepulses ranging from −140 mV to 0 mV in 10-mV increments from a Vmax holding potential. Representative INa traces in both the absence and presence of APAS are shown in A-1, B-1, C-1, and D-1. Effects of APAS on inactivation curves (closed circles, control; open circles, neurosteroids; cross, washout) are shown in A-2, B-2, C-2, and D-2. Steady-state inactivation curves were fitted to the Boltzmann equation, and the V1/2 values are shown in table 2. Data are expressed as means ± SEM. Nav = voltage-gated sodium channel; Wash = washout.

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